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Development of BIO diagnostic kit
As the medical paradigm shifts from treatment to prevention, the importance of the in vitro diagnostic market and its early diagnosis is spotlighted.Based on its own original technology in the field of diagnosis,
NDFOS actively research and develops for technological innovation and globalization in the field of genome diagnosis.
What is Molecular Diagnosis?
Molecular diagnosis uses a small amount of specimens (blood, saliva, urine, etc.) from the human body to detect changes
in the molecular level that occur in DNA/RNA containing genetic information such as bacteria and viruses, and determine infection with high accuracy.
The market continues to demand diagnostic methods with high accuracy and sensitivity,
and NDFOS is focusing on the development and production of diagnostic kits by quickly responding to market demands.
NDFOS Molecular Diagnosis
Consists of experts in DNA replication and enzyme technology, which are the core technology of molecular diagnosis
Independent production of key enzymes for molecular diagnostics and bio-reagents
Gene recombination technology
Protein expression technology
Protein Assay Technology
Protein purification technology
DNA/RNA synthesis technology
Molecular Diagnosis Technology
Multiplex PCR
This is a method of amplifying multiple target genes in the same reaction solution by a polymerase chain reaction to amplify several different DNA sequences at the same time. In this case, the interaction of the primers should be calculated to prevent the primers from forming dimers or interfering with each other, and the amplified products should be different in sizes to be distinguished on the Agarose Gel.
Real-time PCR
As an experimental method based on polymerase chain reaction, it is a method that can calculate the initial amount of DNA present in the sample by monitoring the product amplified during the PCR process in real time. Since it is a method that simultaneously amplifies DNA molecules and measures the amount, the detection sensitivity is high, so it is a very useful molecular biological technology for the analysis of gene mutations such as detection of trace pathogens, quantification of gene expression (mRNA expression), and analysis of single nucleotide polymorphism (SNP). Real Time PCR detects the amount of PCR amplified products using fluorescence, and SYBR Green fluorescent materials and TaqMan Probe are typically used. SYBR Green fluorescence is a substance that shows fluorescence by binding to a DNA double helix. It has the advantage of being inexpensive, but it has the disadvantage of reacting to non-specific amplification products. To overcome this, the melting curve of the amplified DNA product is measured after the amplification to determine whether a non-specific amplification has occurred.NDFOS Molecular Diagnostic Kit
ND COVID-19 Detection Kit We have completed the development of a kit for diagnosing the Novel Coronavirus , which started in Wuhan in 2019,and we are developing and producing ND COVID-19 diagnostic kits to help prevent the spread of COVID-19.
Virus Molecular Diagnostic Kit for SARS-CoV-2 (COVID-19)
Virus Molecular Diagnostic Kit for SARS-CoV-2 is a one-step RT PCR kit that detects RdRp and E genes causing novel coronavirus infection. Two types of Primer Mix per sample makes it possible to classify and detect Beta CoV and SARS-CoV-2.
RdRp Mix especially detects SARS-COV-2, while E gene Mix detects both Beta CoV and SARS CoV-2.
This product contains enzymes and reaction components necessary for cDNA synthesis and PCR amplification, and proceeds mixture of RNA Template, Primer, Probe (Primermix) in one-step to reduce contamination and easily detect RT-PCR reaction. In addition, the use of Taqman Probe enables more accurate and faster analysis.
SARS-CoV-2 (COVID-19) Test Result
· High Sensitivity / High Specificity
· High Accuracy / Rapid
Development and production of raw materials
NDFOS is developing its own polymerases and high-purity recombinant enzymes suitable for the fields of life science and molecular diagnostics on its own,and is carrying out perfect production through strict quality control at the kGMP production facility. NDFOS will stand as a major enzyme supplier in the domestic and international markets,
and contribute to maintenance and improvement of human health through the development of innovative protein drugs
and therapeutic enzymes which will greatly contribute to human health and well-being.
LIST of raw materials that can be produced
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RNase Inhibitor
cDNA Synthesis Kit
2X qPCR Mix (SYBR Green)
T4 DNA Ligase
Phosphatase (Antarctic)
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Taq DNA Polymerase
HOT DNA Polymerase
High Fidelity Polymerase
Pfu DNA Polymerase
Pfu-plus DNA Polymerase
MMLV Reverse Transcriptase
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2X qPCR Mix (TaqMan)
2X One Step RT-PCR Mix (SYBR Green)
2X One Step RT-PCR Mix (TaqMan)
2X One Step RT-PCR Mix with Loading Dye
Klenow DNA Polymerase (DNA polymerase I large fragment)
Klenow DNA Polymerase (3'→5' exo-)
Quotation and contact
If you have any inquiries and need to get a quotation from us, please send an with some information below
